The liquid phase separation chromatograph is generally composed of a constant current pump, a chromatography column, a detector, a data recording and a collector, wherein the chromatography column and the detector are key parts. So how do these devices work together in the process of using them?
The working principle of liquid chromatography chromatograph:
The system of the liquid chromatograph consists of a constant current pump, a column, a detector, a recorder, and a collector. The mobile phase in the accumulator is driven into the column by a constant current pump. The sample solution is chromatographed into the detector and can be collected by the data recorder in real time until the data is desired.
Since the components in the sample solution have different partition coefficients in the two phases, when the two phases are relatively moved, after repeated multiple adsorption-desorption processes, the components have a large moving speed. The difference is separated into individual components and sequentially flowed out of the column. When passing through the detector, the sample concentration is converted into an electrical signal and transmitted to the recorder, and the data is printed in the form of a map.
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