Oxygen electrode experiment preparation
Commonly used appliances:
Scissors (surgical scissors), tweezers (preferably ophthalmology), disposable pipettes, absorbent paper (sanitary rolls), pipettes (pipettes) (1 mL), 1.5 mL centrifuge tubes (10), Beakers two 50mL, 250mL each, wash the bottle (deionized water or distilled water).
Equipped with solution:
Semi-saturated potassium chloride solution: (Use 50mL small beaker 20mL water, add potassium chloride, stir until no longer dissolved, pour the supernatant into another beaker, then add the same amount of water to be semi-saturated chlorination Potassium solution) or weigh 1.86 g of KCl dissolved in 50 ml of distilled water
If it is a Chlorolab2 type oxygen electrode, or to measure photosynthetic oxygen evolution, you need to prepare 50-100ml of 0.1M sodium bicarbonate solution.
Prepare the necessary buffer according to the experimental needs.
drug:
Low sodium sulfite (safety powder), molecular formula: NaO2SSO2Na
Liquid oxygen determination systems are widely used in plant physiology, agronomy, horticulture, forestry, microbiology, algae biology, life sciences, marine biology, zoology, human medicine, and environmental science.
The liquid oxygen measurement system has the following important features:
1) It is commonly used to study the photosynthesis rate of various animal, plant and microbial tissue respiration rate, plants, algae, etc. It is also used to study the changes of respiratory rate and respiratory pathway of plant tissues, and analyze cyanide-resistant respiratory pathway and cytochrome oxidase pathway. Changes in the glycolytic pathway and the tricarboxylic acid pathway, and then the changes in plant stress resistance, tissue dormancy and dormancy release.
2) Determination of respiratory and I state, II state, III state, IV state respiration of mitochondria in animals, plants, etc., study respiratory control rate and P/O ratio
3) Determination of the enzymatic reaction process involving aerobics. Such as polyphenol oxidase, lipoxygenase, H2O2 enzyme and other activities
4) Determine the rate of change of oxygen during the chemical reaction.
5) It can be combined with 8 systems to control each system through one computer.
The real-time dissolved oxygen changes in the 8 reaction chambers were monitored.
Can be used with OXY/PHA ion selective pH electrode amplifiers.
At the same time, the changes of oxygen concentration and H+ concentration in the reaction solution were detected.
Difference between oxygen electrode models
1) Oxygraph can be controlled by a circulating water bath, but an additional water bath is required (the one must have circulating water)
2) Oxygtherm is equipped with an integrated electronic temperature control device, which can accurately control the temperature between 3 °C and 40 °C, and the temperature control accuracy is 0.02 °C. This avoids the use of a super constant temperature water bath. Oxytherm can be used to study the oxygen consumption process of various biological materials such as animals, plants, microorganisms and algae.
3) Chlorolab-2 and Chlorolab-3 oxygen electrodes have a light source, and the sample chamber has a circulating water bath interlayer, which can be controlled by an external circulating water bath.
The oxygen- and oxygen-consuming processes can be measured by the Chlorolab-2 and Chlorolab-3 oxygen electrodes.
The Chlorolab-2 and Chlorolab-3 oxygen electrodes can be combined with HANSATECH's FMS 2 fluorescence instrument to measure photosynthetic oxygen evolution rate and chlorophyll fluorescence.
4) Chlorolab-2 three light quality (white, red, blue) LED light source maximum nominal light intensity reduced from 3000μmol·M-2·S-1 to 2000μmol·M-2·S-1
The nominal maximum intensity of Chlorolab-3 red LED light source is reduced from 1200μmol·M-2·S-1 to 900μmol·M-2·S-1
The Oxytherm+P reaction chamber contains a white LED light source with a maximum light intensity of 4000μmol·M-2·S-1. There is a visor to darken the sample chamber to determine the respiration rate.
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