Experimental principle of human P glycoprotein / permeable glycoprotein (P-gp) ELISA kit
English name: Human permeability glycoprotein, P-gp ELISA
1. The level of human P-glycoprotein / permeable glycoprotein (P-gp) ELISAKit was determined by double antibody sandwich method.
2. First coat the microplate with purified human P glycoprotein / permeable glycoprotein (P-gp) ELISAKit antibody to make a solid phase antibody.
3. Then add P glycoprotein / permeable glycoprotein (P-gp) ELISAKit to the microwells coated with mAb in turn, and then combine with HRP-labeled P glycoprotein / permeable glycoprotein (P-gp) ELISAKit antibody, An antibody-antigen-enzyme-labeled antibody complex is formed, and after thorough washing, a substrate TMB is added for color development. TMB is converted into blue under the catalysis of HRP enzyme, and into the final yellow under the action of acid.
4. The color depth is positively correlated with the P glycoprotein / permeable glycoprotein (P-gp) ELISAKit in the sample.
5. Finally, the absorbance (OD value) is measured with a microplate reader at a wavelength of 450 nm, and the concentration of human P glycoprotein / permeable glycoprotein (P-gp) ELISAKit in the sample is calculated from the standard curve
.
Objective: To determine the content of P-glycoprotein / permeable glycoprotein (P-gp) ELISAKit in human serum, plasma and related liquid samples.
6. Serum: Blood coagulates naturally at room temperature for 10-20 minutes, centrifuged for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully and centrifuge again if a precipitate appears during storage. Avoid any cell stimulation during the operation. Use test tubes free of pyrogens and endotoxins.
7. Plasma: EDTA or sodium citrate should be selected as the anticoagulant according to the requirements of the specimen. After mixing for 10-20 minutes, centrifuge for about 20 minutes (2000-3000 rpm) to remove particles. Collect the supernatant carefully, and centrifuge again if there is any precipitate.
safety:
1. Avoid direct contact with stop solution and substrate. If you accidentally come into contact with these liquids, please rinse them with water as soon as possible.
2. Do not eat, drink, smoke or use cosmetics during the experiment.
3. Do not touch any ingredients in the kit with your mouth.
Outdoor sofa is a kind of furniture for people to rest, relax and entertain in the outdoors. They are usually made of durable materials that can withstand the effects of various climatic conditions. The classification of outdoor sofas is mainly based on materials, design and function.
First, material classification:
1. Wooden outdoor sofa: This type of outdoor sofa is usually made of natural wood, such as teak. They are naturally beautiful and durable, suitable for outdoor Spaces such as gardens and terraces.
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3. Artificial materials outdoor sofa: This kind of outdoor sofa is usually made of artificial materials, such as plastic, artificial wood and so on. They have the characteristics of light, easy to clean, suitable for outdoor restaurants, cafes and other commercial places.
Ii. Design classification:
1. Single outdoor sofa: This kind of outdoor sofa is simple in design and suitable for single use. They are usually small and lightweight, suitable for small balconies, garden corners and other places where space is limited.
2. Double outdoor sofa: This kind of outdoor sofa is designed to be comfortable and suitable for two people. They are usually more spacious and suitable for placing in larger Spaces such as terraces and courtyards.
3. Multi-person outdoor sofa: This type of outdoor sofa is spacious and suitable for multi-person use. They often function as a sofa bed and can be used as a makeshift bed. Suitable for outdoor banquet area, holiday villa and other large outdoor space.
Iii. Functional classification:
1. Leisure outdoor sofa: This kind of outdoor sofa design is comfortable, suitable for people to relax in the outdoors. They are usually equipped with soft cushions and cushions to provide comfortable support.
2. Dining outdoor sofa: This type of outdoor sofa is designed for outdoor dining, such as open-air restaurants, cafes, etc. They are usually equipped with a dining table and chairs, providing a convenient dining experience.
Of course, a variety of different sofas can also be freely combined into a sofa combination to meet your different needs.
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