The density gradient separation method is a commonly used method for separating small biological molecules. It is widely used in the separation and purification of viruses, the separation of subcellular organelles, proteins, plasmid DNA, RNA, etc. The most notable feature is the high purity of the samples obtained.
The density gradient centrifugation experiment includes three main steps: gradient preparation, centrifugal separation, and sample collection. The centrifugal separation part is completed by an ultracentrifuge, while the gradient preparation and sample separation are still at the stage of manual operation. The two steps are common It is characterized by complicated operation, long time consumption, manual operation and poor repeatability. This is a common annoyance for many experiment operators, and it is also a major bottleneck of ultracentrifugation, that is, the gradient linearity is poor and sample collection is difficult.
The automatic density gradient preparation and separation system can easily solve this problem.
Full automatic density gradient separation process
Canada Biocomp automatic density gradient preparation
Take the density gradient of 5% -20% sucrose solution as an example:
The Biocomp automatic gradient preparation device has a built-in program that can set the gradient preparation speed, time, and angle. It can be connected to a computer to upload and download the gradient preparation program. It can be used for gradient preparation of various solutions, including SOUCROSE, GLYCEROL, OPTIPREP, NYCODENZ, FICOLL, PERCOLL, METRIZAMIDE, RENOGRAFIN, NaCl, CsCl, KCl and other gradient media.
Canada Biocomp automatic density gradient separator
Biocomp automatic density gradient separator has automatic cleaning function, which can be programmed or manually clean and dry the pipeline to avoid cross contamination.
In addition, the Biocomp density gradient separator can also be connected to the UV detector and fraction collector to achieve online real-time detection and collection of samples. The standard software is connected to the computer to display the signal acquisition process, data superposition, processing and other functions in real time And export the data in exl format.
Biocomp also specially set up a gradient preparation and separation integrated machine to realize the all-round processing of gradient preparation.
Biocomp automatic density gradient system has obvious advantages of fast, efficient and standardized, which greatly improves the efficiency of density gradient separation, saves valuable experimental time, and is recognized and loved by more and more scientific researchers.
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