Experimental principle of ELISA kit for human tumor necrosis factor-related apoptosis-inducing ligand 3 (TRAIL-R3)

1. The level of human tumor necrosis factor-related apoptosis-inducing ligand 3 (TRAIL-R3) ELISAKit was determined by double antibody sandwich method.

2. First, coat the microplate with purified human tumor necrosis factor-related apoptosis-inducing ligand 3 (TRAIL-R3) ELISAKit antibody to prepare a solid-phase antibody.

3. Then add the tumor necrosis factor-related apoptosis inducing ligand 3 (TRAIL-R3) ELISAKit to the microwells coated with mAb in turn, and then add HRP-labeled tumor necrosis factor-related apoptosis inducing ligand 3 (TRAIL-R3) The ELISAKit antibody binds to form an antibody-antigen-enzyme-labeled antibody complex. After thorough washing, the substrate TMB is added for color development. TMB is converted into blue under the catalysis of HRP enzyme, and into the final yellow under the action of acid.

4. The color depth is positively correlated with the tumor necrosis factor-related apoptosis-inducing ligand 3 (TRAIL-R3) ELISAKit in the sample.

5. Finally, the absorbance (OD value) is measured at 450nm wavelength using a microplate reader, and the concentration of human tumor necrosis factor-related apoptosis-inducing ligand 3 (TRAIL-R3) ELISAKit concentration in the sample is calculated from the standard curve

Objective: To determine the content of tumor necrosis factor-related apoptosis-inducing ligand 3 (TRAIL-R3) ELISAKit in human serum, plasma and related liquid samples.

6. Serum: Blood coagulates naturally at room temperature for 10-20 minutes, centrifuged for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully and centrifuge again if a precipitate appears during storage. Avoid any cell stimulation during the operation. Use test tubes free of pyrogens and endotoxins.

7. Plasma: EDTA or sodium citrate should be selected as the anticoagulant according to the requirements of the specimen. After mixing for 10-20 minutes, centrifuge for about 20 minutes (2000-3000 rpm) to remove particles. Collect the supernatant carefully, and centrifuge again if there is any precipitate.

safety:
1. Avoid direct contact with stop solution and substrate. If you accidentally come into contact with these liquids, please rinse them with water as soon as possible.
2. Do not eat, drink, smoke or use cosmetics during the experiment.
3. Do not touch any ingredients in the kit with your mouth.

4 Keep Elisa kit away from children

Bring your own items:

1. 37 â„ƒ incubator

2. Standard specification microplate reader

3. Precision pipette and disposable tip

4. Distilled water

5. Disposable test tube

6. Absorbent paper

Storage conditions and validity period:

1. Kit storage :; 2-8 â„ƒ.

2. Validity: 6 months