Reagent composition Precision microtiter plates 96 wells (Microtitration Strips) 1 piece 2 ～ 8 ℃ dry storage enzyme labeling coupling solution (Conjugate) 1 bottle 12.0 ml 2 ～ 8 ℃ cold storage standard (Standard) 5 bottles 1.0ml each Substrate A (refrigerated at 8 ° C) 1 bottle of 6.0 ml 2 to 8 ° C protected from light, Substrate B (Substrate B) 1 vial (stopped solution) of 6.0 ml of 2 to 8 ° C protected from light 1 bottle of 6.0 ml at room temperature
20 times concentrated washing solution (Rinsing Buffer x 20) 1 bottle of 60.0 ml 2 ～ 8 ℃
5 times concentrated sample diluent (Diluent x 5) 1 bottle of 15.0ml 2 ～ 8 ℃ refrigerated and stored in English manual, one Chinese manual at room temperature
2. Matters needing attention
1. This reagent is an in vitro test reagent. It should be used within the validity period. The reagent should be regarded as an infectious agent.
The reagents in the box should be taken out before use. Leave at room temperature for at least 30 minutes,
After the concentrated washing solution crystallizes, incubate at 37 ° C for 15 minutes.
After the concentrated sample diluent crystallizes, please incubate at 37 ℃ for 15 minutes. If the experiment is performed within 24 hours, the specimen can be stored at 2 ～ 8 ℃. No need for timely experiment, the specimen should be stored at -20 ℃ to avoid repeated freezing and thawing.
After repeatedly cleaning the microwell plate, and buckle the residual liquid in the microwell, otherwise it will reduce the accuracy and cause the false image of absorbance deviation.
After the sample is added, the microwell reaction strip should be shaken slightly to mix the liquid in the well.
The kit is stored at 2 ～ 8 ℃, please do not freeze it, please refer to the label in the box for the expiration date.
3. Preparation before the experiment
1. Before use, the reagents in the box should be taken out and left at room temperature for at least 30 minutes.
2. Prepare various experimental instruments and materials, such as micropipettes, pipette tips, medical distilled water, etc.
3. Concentrated lotion and medical distilled water are diluted 1:19 times to become an application lotion
4. 4. Dilute the concentrated sample diluent and medical distilled water 1: 4 times into the applied sample diluent 5． Dilute the sample with the application sample diluent, dilute the sample according to the volume ratio of 1: 100, for example, 10μl of the sample is added to the 1ml application sample diluent, and mix well to use. )
4. Operation steps Take out the microtiter plate, set up blank wells, and add 100μl of the standard products to the blank micro-wells according to the order (the blank wells are regarded as No. 0 standards, and they are replaced with medical distilled water)
2. Mark the sample number separately and add 100 μl of the diluted sample to the blank microwell (different samples use different tips).
3. Incubate the microplate at 37 ° C for 30 minutes;
4. Take out the enzyme labeling plate and shake off the liquid in it. After filling all the holes with the application washing liquid, immediately shake off the liquid;
5. After the application washing solution is filled in each well, shake the enzyme plate slightly for 30 seconds, then shake off the application washing solution in the well, and pat the enzyme plate dry on the absorbent paper.
6. Repeat the fifth step 5 times, pat the enzyme plate dry on absorbent paper.
7. Add 100 μl of enzyme-labeled coupling solution to the standard well and sample well.
8. Incubate the 96-well plate at 37 ° C for 30 minutes.
9. Take out the enzyme labeling plate and shake off the liquid. After all the application washing liquid is filled in each well, immediately shake off the liquid.
10. After refilling each well with the washing application liquid, shake the enzyme plate slightly at room temperature for 30 seconds, then shake off the application washing liquid in the wells and pat the enzyme plate on the absorbent paper to dry.
11. Repeat the fifth step 5 times, pat the enzyme plate on the absorbent paper to dry.
12. Add 50 μl of substrate A to each well immediately after adding 50 μl of substrate B. Gently shake to mix. (Liquid A and B are sampled with different tips)
13. Incubate the microplate at 37 ° C in the dark for 15 minutes.
14. Add 50 μl of stop solution to each microwell and gently shake to mix.
15. Measure OD at 450nm on a microplate reader; after color development, measure within 15 minutes.
16. Calculate the sample content according to the prepared standard curve
V. Judgment of results 1. Instrument value: read the OD value of each well on a microplate reader with a wavelength of 450 nm;
2. Range of detection value: 0-40ng / ml
3. Sensitivity: 0.1ng / ml
Outdoor furniture refers to furniture used for outdoor environment, mainly used for outdoor leisure, entertainment and dining activities. The classification of outdoor furniture can be divided according to the material, function and shape.
First, classification by material
1. Wooden outdoor furniture: Wooden outdoor furniture is usually made of natural wood, such as teak. Wooden furniture has a natural, rustic character and is suitable for outdoor Spaces such as gardens, terraces and courtyards.
2. Metal outdoor furniture: Metal outdoor furniture is usually made of iron, aluminum, stainless steel and other metal materials. Metal furniture has the characteristics of durability and stability, suitable for long-term use in outdoor environment.
3. Cloth outdoor furniture: Cloth outdoor furniture is usually made of waterproof, sun protection and other special treatment of fabric. Fabric furniture with soft, comfortable characteristics, suitable for use in outdoor leisure areas.
Second, Classification by function
1. Leisure Chair: Leisure chair is the most common kind of outdoor furniture, usually used for outdoor leisure, sunbathing and other activities. There are a variety of leisure chairs, folding chairs, recliners, rocking chairs and so on.
2. Dining Table And Chair: Dining table and chair are mainly used for outdoor dining, usually consisting of a table and several chairs. Dining tables and chairs of various styles, there are round tables, square tables, etc., suitable for different venues and the number of people dining needs.
3. Parasol: Parasol is used for outdoor shading, which can effectively block direct sunlight and provide a cool resting environment. There are various styles of sunshade umbrellas, including ordinary umbrellas, large umbrellas, and rotating umbrellas.
Third, Classification by shape
1. Chairs: including ordinary chairs, rocking chairs, recliners, etc., suitable for outdoor leisure and sun.
2. Table category: including dining table, coffee table, side table, etc., suitable for outdoor dining and placing items.
3. Sofa category: including single sofa, double sofa, combination sofa, etc., suitable for outdoor leisure and parties.
4. Reclining chairs: including hammocks, reclining chairs, etc., suitable for outdoor naps and rest.
5. Queue class: including benches, benches, etc., suitable for outdoor queuing and waiting.
In summary, the classification of outdoor furniture can be divided according to material, function and shape. Different outdoor furniture is suitable for different outdoor environment and activity needs, choose suitable outdoor furniture can provide comfortable and convenient outdoor life experience.
Outdoor Table,Rattan Table,Rattan Chair,Beach Furniture
Foshan Shengshi Zhihui Technology Co., Ltd. , https://www.officeroutdoorfurniture.com